Virus:Article Title: Protein kinase R and the integrated stress response drive immunopathology caused by mutations in the RNA deaminase ADAR1.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse monoclonal anti-b Actin SIGMA cat # A5441 ADAR1 Antibody (15.8.6) Santa Cruz Biotechnology cat # sc-73408 Bacterial and virus strains Encephalomyocarditis virus (EMCV)-K Gorman et al., 2017 N/A Chemicals, peptides, and recombinant proteins 2BAct mouse chow Wong et al., 2019 N/A Envigo Teklad Global 14% Protein Rodent Maintenance Diet Envigo Cat # 2914 BioServ Rodent Reproductive Diet Bio Serv Product# S3823P Recombinant mouse Interferon beta protein R&D Systems Cat # 12405-1 Recombinant human interferon beta protein R&D Systems Cat # 11415-1 ISRIB SIGMA Cat # SML0843 Critical commercial assays Taq Man Gene Expression Assays Thermo Fisher Cat # 4351372 Deposited data RNA Seq raw data of P195A/p150+ and P195A/p150- liver, kidney, and cerebellum GEO GSE162583 Experimental models: Cell lines C57BL/6 primary MEFs, (mouse embryonic fibroblasts) Gray et al., 2015 N/A HEK293T cells ATCC CRL-3216 A549 cells ATCC CCL-185 mouse bone marrow macrophages, C57BL/6J Gray et al., 2015 N/A Experimental models: Organisms/strains mouse: Adar P195A: C57BL/6J this paper N/A mouse: Eif2ak2 / : C57BL/6JEif2ak2tm1Jcbe Nakamura et al., 2015 N/A mouse: Adar p150+/ : C57BL/6J Pestal et al., 2015 N/A mouse: Adar+/ : C57BL/6J Pestal et al., 2015 N/A mouse: Dhx58 / : C57BL/6J-Dhx58tm1Itl Suthar et al., 2012 N/A mouse: Ifih1 / : C57BL/6J-Ifih1tm1.1Cln Gitlin et al., 2006 N/A mouse: Rnasel / : C57BL/6J-Rnaseltm1Slvm Zhou et al., 1997 N/A mouse: Ifnar1 / : C57BL/6J-Ifnar1tm1Agt Kolumam et al., 2005 N/A Oligonucleotides See Table S1 for qPCR primer sequences See Table S1 N/A See Table S2 for guide RNA sequences See Table S2 N/A Recombinant DNA pRRL lentiCRISPR vectors. .. Guide RNA sequences in Table S2 Gray et al., 2016 N/A Software and algorithms Prism v 9.0 GraphPad N/A Adobe Photoshop Elements 2020 Adobe N/A Galaxy FASTQ Trimmer tool v1.0.0 N/A N/A (Continued on next page) Immunity 54, 1948–1960.e1–e5, September 14, 2021 e1
Recombinant:Article Title: Protein kinase R and the integrated stress response drive immunopathology caused by mutations in the RNA deaminase ADAR1.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse monoclonal anti-b Actin SIGMA cat # A5441 ADAR1 Antibody (15.8.6) Santa Cruz Biotechnology cat # sc-73408 Bacterial and virus strains Encephalomyocarditis virus (EMCV)-K Gorman et al., 2017 N/A Chemicals, peptides, and recombinant proteins 2BAct mouse chow Wong et al., 2019 N/A Envigo Teklad Global 14% Protein Rodent Maintenance Diet Envigo Cat # 2914 BioServ Rodent Reproductive Diet Bio Serv Product# S3823P Recombinant mouse Interferon beta protein R&D Systems Cat # 12405-1 Recombinant human interferon beta protein R&D Systems Cat # 11415-1 ISRIB SIGMA Cat # SML0843 Critical commercial assays Taq Man Gene Expression Assays Thermo Fisher Cat # 4351372 Deposited data RNA Seq raw data of P195A/p150+ and P195A/p150- liver, kidney, and cerebellum GEO GSE162583 Experimental models: Cell lines C57BL/6 primary MEFs, (mouse embryonic fibroblasts) Gray et al., 2015 N/A HEK293T cells ATCC CRL-3216 A549 cells ATCC CCL-185 mouse bone marrow macrophages, C57BL/6J Gray et al., 2015 N/A Experimental models: Organisms/strains mouse: Adar P195A: C57BL/6J this paper N/A mouse: Eif2ak2 / : C57BL/6JEif2ak2tm1Jcbe Nakamura et al., 2015 N/A mouse: Adar p150+/ : C57BL/6J Pestal et al., 2015 N/A mouse: Adar+/ : C57BL/6J Pestal et al., 2015 N/A mouse: Dhx58 / : C57BL/6J-Dhx58tm1Itl Suthar et al., 2012 N/A mouse: Ifih1 / : C57BL/6J-Ifih1tm1.1Cln Gitlin et al., 2006 N/A mouse: Rnasel / : C57BL/6J-Rnaseltm1Slvm Zhou et al., 1997 N/A mouse: Ifnar1 / : C57BL/6J-Ifnar1tm1Agt Kolumam et al., 2005 N/A Oligonucleotides See Table S1 for qPCR primer sequences See Table S1 N/A See Table S2 for guide RNA sequences See Table S2 N/A Recombinant DNA pRRL lentiCRISPR vectors. .. Guide RNA sequences in Table S2 Gray et al., 2016 N/A Software and algorithms Prism v 9.0 GraphPad N/A Adobe Photoshop Elements 2020 Adobe N/A Galaxy FASTQ Trimmer tool v1.0.0 N/A N/A (Continued on next page) Immunity 54, 1948–1960.e1–e5, September 14, 2021 e1
Gene Expression:Article Title: Protein kinase R and the integrated stress response drive immunopathology caused by mutations in the RNA deaminase ADAR1.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse monoclonal anti-b Actin SIGMA cat # A5441 ADAR1 Antibody (15.8.6) Santa Cruz Biotechnology cat # sc-73408 Bacterial and virus strains Encephalomyocarditis virus (EMCV)-K Gorman et al., 2017 N/A Chemicals, peptides, and recombinant proteins 2BAct mouse chow Wong et al., 2019 N/A Envigo Teklad Global 14% Protein Rodent Maintenance Diet Envigo Cat # 2914 BioServ Rodent Reproductive Diet Bio Serv Product# S3823P Recombinant mouse Interferon beta protein R&D Systems Cat # 12405-1 Recombinant human interferon beta protein R&D Systems Cat # 11415-1 ISRIB SIGMA Cat # SML0843 Critical commercial assays Taq Man Gene Expression Assays Thermo Fisher Cat # 4351372 Deposited data RNA Seq raw data of P195A/p150+ and P195A/p150- liver, kidney, and cerebellum GEO GSE162583 Experimental models: Cell lines C57BL/6 primary MEFs, (mouse embryonic fibroblasts) Gray et al., 2015 N/A HEK293T cells ATCC CRL-3216 A549 cells ATCC CCL-185 mouse bone marrow macrophages, C57BL/6J Gray et al., 2015 N/A Experimental models: Organisms/strains mouse: Adar P195A: C57BL/6J this paper N/A mouse: Eif2ak2 / : C57BL/6JEif2ak2tm1Jcbe Nakamura et al., 2015 N/A mouse: Adar p150+/ : C57BL/6J Pestal et al., 2015 N/A mouse: Adar+/ : C57BL/6J Pestal et al., 2015 N/A mouse: Dhx58 / : C57BL/6J-Dhx58tm1Itl Suthar et al., 2012 N/A mouse: Ifih1 / : C57BL/6J-Ifih1tm1.1Cln Gitlin et al., 2006 N/A mouse: Rnasel / : C57BL/6J-Rnaseltm1Slvm Zhou et al., 1997 N/A mouse: Ifnar1 / : C57BL/6J-Ifnar1tm1Agt Kolumam et al., 2005 N/A Oligonucleotides See Table S1 for qPCR primer sequences See Table S1 N/A See Table S2 for guide RNA sequences See Table S2 N/A Recombinant DNA pRRL lentiCRISPR vectors. .. Guide RNA sequences in Table S2 Gray et al., 2016 N/A Software and algorithms Prism v 9.0 GraphPad N/A Adobe Photoshop Elements 2020 Adobe N/A Galaxy FASTQ Trimmer tool v1.0.0 N/A N/A (Continued on next page) Immunity 54, 1948–1960.e1–e5, September 14, 2021 e1
RNA Sequencing:Article Title: Protein kinase R and the integrated stress response drive immunopathology caused by mutations in the RNA deaminase ADAR1.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse monoclonal anti-b Actin SIGMA cat # A5441 ADAR1 Antibody (15.8.6) Santa Cruz Biotechnology cat # sc-73408 Bacterial and virus strains Encephalomyocarditis virus (EMCV)-K Gorman et al., 2017 N/A Chemicals, peptides, and recombinant proteins 2BAct mouse chow Wong et al., 2019 N/A Envigo Teklad Global 14% Protein Rodent Maintenance Diet Envigo Cat # 2914 BioServ Rodent Reproductive Diet Bio Serv Product# S3823P Recombinant mouse Interferon beta protein R&D Systems Cat # 12405-1 Recombinant human interferon beta protein R&D Systems Cat # 11415-1 ISRIB SIGMA Cat # SML0843 Critical commercial assays Taq Man Gene Expression Assays Thermo Fisher Cat # 4351372 Deposited data RNA Seq raw data of P195A/p150+ and P195A/p150- liver, kidney, and cerebellum GEO GSE162583 Experimental models: Cell lines C57BL/6 primary MEFs, (mouse embryonic fibroblasts) Gray et al., 2015 N/A HEK293T cells ATCC CRL-3216 A549 cells ATCC CCL-185 mouse bone marrow macrophages, C57BL/6J Gray et al., 2015 N/A Experimental models: Organisms/strains mouse: Adar P195A: C57BL/6J this paper N/A mouse: Eif2ak2 / : C57BL/6JEif2ak2tm1Jcbe Nakamura et al., 2015 N/A mouse: Adar p150+/ : C57BL/6J Pestal et al., 2015 N/A mouse: Adar+/ : C57BL/6J Pestal et al., 2015 N/A mouse: Dhx58 / : C57BL/6J-Dhx58tm1Itl Suthar et al., 2012 N/A mouse: Ifih1 / : C57BL/6J-Ifih1tm1.1Cln Gitlin et al., 2006 N/A mouse: Rnasel / : C57BL/6J-Rnaseltm1Slvm Zhou et al., 1997 N/A mouse: Ifnar1 / : C57BL/6J-Ifnar1tm1Agt Kolumam et al., 2005 N/A Oligonucleotides See Table S1 for qPCR primer sequences See Table S1 N/A See Table S2 for guide RNA sequences See Table S2 N/A Recombinant DNA pRRL lentiCRISPR vectors. .. Guide RNA sequences in Table S2 Gray et al., 2016 N/A Software and algorithms Prism v 9.0 GraphPad N/A Adobe Photoshop Elements 2020 Adobe N/A Galaxy FASTQ Trimmer tool v1.0.0 N/A N/A (Continued on next page) Immunity 54, 1948–1960.e1–e5, September 14, 2021 e1
Real-time Polymerase Chain Reaction:Article Title: Protein kinase R and the integrated stress response drive immunopathology caused by mutations in the RNA deaminase ADAR1.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse monoclonal anti-b Actin SIGMA cat # A5441 ADAR1 Antibody (15.8.6) Santa Cruz Biotechnology cat # sc-73408 Bacterial and virus strains Encephalomyocarditis virus (EMCV)-K Gorman et al., 2017 N/A Chemicals, peptides, and recombinant proteins 2BAct mouse chow Wong et al., 2019 N/A Envigo Teklad Global 14% Protein Rodent Maintenance Diet Envigo Cat # 2914 BioServ Rodent Reproductive Diet Bio Serv Product# S3823P Recombinant mouse Interferon beta protein R&D Systems Cat # 12405-1 Recombinant human interferon beta protein R&D Systems Cat # 11415-1 ISRIB SIGMA Cat # SML0843 Critical commercial assays Taq Man Gene Expression Assays Thermo Fisher Cat # 4351372 Deposited data RNA Seq raw data of P195A/p150+ and P195A/p150- liver, kidney, and cerebellum GEO GSE162583 Experimental models: Cell lines C57BL/6 primary MEFs, (mouse embryonic fibroblasts) Gray et al., 2015 N/A HEK293T cells ATCC CRL-3216 A549 cells ATCC CCL-185 mouse bone marrow macrophages, C57BL/6J Gray et al., 2015 N/A Experimental models: Organisms/strains mouse: Adar P195A: C57BL/6J this paper N/A mouse: Eif2ak2 / : C57BL/6JEif2ak2tm1Jcbe Nakamura et al., 2015 N/A mouse: Adar p150+/ : C57BL/6J Pestal et al., 2015 N/A mouse: Adar+/ : C57BL/6J Pestal et al., 2015 N/A mouse: Dhx58 / : C57BL/6J-Dhx58tm1Itl Suthar et al., 2012 N/A mouse: Ifih1 / : C57BL/6J-Ifih1tm1.1Cln Gitlin et al., 2006 N/A mouse: Rnasel / : C57BL/6J-Rnaseltm1Slvm Zhou et al., 1997 N/A mouse: Ifnar1 / : C57BL/6J-Ifnar1tm1Agt Kolumam et al., 2005 N/A Oligonucleotides See Table S1 for qPCR primer sequences See Table S1 N/A See Table S2 for guide RNA sequences See Table S2 N/A Recombinant DNA pRRL lentiCRISPR vectors. .. Guide RNA sequences in Table S2 Gray et al., 2016 N/A Software and algorithms Prism v 9.0 GraphPad N/A Adobe Photoshop Elements 2020 Adobe N/A Galaxy FASTQ Trimmer tool v1.0.0 N/A N/A (Continued on next page) Immunity 54, 1948–1960.e1–e5, September 14, 2021 e1
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